Histone H2A.X Rabbit mAb

263365

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N263365

Product Name: Anti-Histone H2A.X Rabbit Monoclonal Antibody

Synonyms: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X

UNIPROT ID: P16104

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Immunogen: A synthetic peptide of human Histone H2A.X

Applications: WB,IHC-F,IHC-P,ICC/IF,IP

Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Host Species: Rabbit

Clonality: Rabbit Monoclonal

Clone ID: R02-3C8

MW: Calculated MW: 15 kDa; Observed MW: 15 kDa

Isotype: IgG

Purification: Affinity Purified

Species Reactivity: Human

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: EpigeneticsyyyyyHistone methylation

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.	Immunocytochemistry analysis of Histone H2A.X (green) in Hela using Histone H2A.X antibody,and DAPI(blue).
Western blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X antibody.

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