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CD70 Rabbit pAb

221123

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S221123
Product Name:   Anti-CD70 Rabbit Polyclonal Antibody
Synonyms:   CD27L; LPFS3; CD27-L; CD27LG; TNFSF7; TNLG8A
UNIPROT ID:   P32970 (Gene Accession - NP_001243 )
Background:    The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
Immunogen:    Synthetic peptide of human CD70
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 221123(CD70 Antibody) at a dilution of 1/60(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 221123(Anti-CD70 Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: A172 cell lysate;
Primary antibody: 221123(CD70 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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