GPRC5D (DM60) Rabbit mAb

28363

Size：100 μg Price：$ 445 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 28363

Product Name: Anti-GPRC5D(DM60) Rabbit Monoclonal Antibody

Synonyms: GPRC5D

Description: Anti-GPRC5D antibody(DM60) Rabbit Monoclonal Antibody

Background: The protein encoded by this gene is a member of the G protein-coupled receptor family; however; the specific function of this gene has not yet been determined.

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human GPRC5D

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human GPRC5D protein, hFc-His tagged protein 11189 can bind Rabbit anti-GPRC5D monoclonal antibody ( clone: DM60) in a linear range of 1-100 ng/ml.	Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Rabbit anti-GPRC5D monoclonal antibody 15µg/ml (clone: DM60) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody ( clone: DM60) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.	Figure 4. Affinity ranking of different Rabbit anti- GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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