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TNFRSF10B (DM113) Rabbit mAb

28458

Size:100 μg Price:$ 445 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   28458
Product Name:   Anti-TNFRSF10B(DM113) Rabbit Monoclonal Antibody
Synonyms:   TNFRSF10B;TRAILR2;TRAIL-R2;CD262;DR5;KILLER;TRICK2;ZTNFR9;TRICKB
Description:    Anti-TNFRSF10B antibody(DM113) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is a member of the TNF-receptor superfamily; and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10:TRAIL:APO-2L); and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD; a death domain containing adaptor protein; is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human TNFRSF10B
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human TNFRSF10B protein, mFc tagged protein 11277 can bind Rabbit anti-TNFRSF10B monoclonal antibody  (clone: DM113) in a linear range of 0.6-90 ng/ml.

Figure 2. TNFRSF10B protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-TNFRSF10B  (DM113) on Expi293 cells transfected with human TNFRSF10B (Blue histogram)or Expi293 transfected with irrelevant protein(Red histogram), and  Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
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