Size：100 μl
Price：$ 219
Brand：NewEast 
Place of Origin：USA
Immunogen：
BRaf Polyclonal Antibody
| Cat. #: 21271 |
| Gene Symbol: B-RAF, B-RAF1, RAFB1 |
| Description: Anti-BRaf Rabbit Polyclonal Antibody |
| Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers. |
| Immunogen: Purified B-Raf protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.1mg/ml |
| Host Species: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes B-Raf of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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