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BRAF(D594G)

26200

Size:100 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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BRAF(D594G)
Cat. #:   26200
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRAF(D594G) Mouse Monoclonal Antibody
Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.
Applications:   ELISA, WB
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D594G mutant, but not wild type BRAF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant BRAF(D594G) and wild type proteins. Purified His-tagged BRAF(D594G) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRAF(D594G) monoclonal antibody (Cat. # 26200).
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