Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
EGFR(T854A)
| Cat. #: 26481 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti-EGFR(T854A) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of T854A, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:200 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR (T854A), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant EGFR (T854A) and wild type proteins.
Purified His-tagged EGFR (T854A) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(T854A) mouse monoclonal antibody (Cat. #26481).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (T854A) antibody.
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(T854A) plasmid (right column), then fixed and stained with anti-EGFR (T854A) monoclonal antibody (Cat. #26481).
Select By Alphabet
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Subscribe to our latest email
(+1) 610-945-2007 [email protected] [email protected] 840 First Avenue, Suite 400, King of Prussia, PA 19406
Copyright © 2010 - 2024 NewEast Biosciences | All rights reserved
Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
Add to cart
Buy now
Download
