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SPI1 Rabbit mAb

263514

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263514
Product Name:   Anti-SPI1 Rabbit Monoclonal Antibody
Synonyms:   Dis1; PU.1; Dis-1; Sfpi1; Spi-1; Sfpi-1; Tcfpu1; Tfpu.1
UNIPROT ID:   P17433
Background:    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
Immunogen:    A synthetic peptide of mouse PU.1/Spi1
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-1D7
MW:    Calculated MW: 31 kDa; Observed MW: 31 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Hamster,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of PU.1/Spi1 (green) in Raw264.7 using PU.1/Spi1 antibody,and DAPI(blue)

Immunohistochemistry analysis of paraffin-embedded mouse liver using SPI1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of PU.1/Spi1 in CHO-K1, C6 lysates using PU.1/Spi1 antibody.
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