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HIF1AN Rabbit mAb

263362

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263362
Product Name:   Anti-HIF1AN Rabbit Monoclonal Antibody
Synonyms:   FIH 1; FIH1; HIF1AN
UNIPROT ID:   Q9NWT6
Background:    Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator.
Immunogen:    A synthetic peptide of human HIF1AN/FIH-1
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-2B3
MW:    Calculated MW: 40 kDa; Observed MW: 40 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Hypoxia Signal Transductionyyyyy Hypoxia-Inhibition
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HIF1AN/FIH1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of HIF1AN (green) in HT-1080 using HIF1AN antibody,and DAPI(blue).

Western blot analysis of HIF1AN/FIH1 in Hela, A549, HL-60, U2OS, U87-MG lysates using HIF1AN/FIH1 antibody.
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