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BMI1 Rabbit mAb

263240

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263240
Product Name:   Anti-BMI1 Rabbit Monoclonal Antibody
Synonyms:   BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51
UNIPROT ID:   P35226
Background:    The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27.
Immunogen:    Recombinant protein of human Bmi1
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-7A6
MW:    Calculated MW: 37 kDa; Observed MW: 43 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Bmi1 in C6, rat Brain, Ramos lysates using Bmi1 antibody

Immunocytochemistry analysis of BMI1 (green) in Hela using BMI1 antibody,and DAPI(blue).

Western blot analysis of Bmi1 in 3T3 lysates using Bmi1 antibody.
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