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Phospho-CDK1/2 (Thr14) Rabbit mAb

263153

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263153
Product Name:   Anti-Phospho-CDK1/2 (Thr14) Rabbit Monoclonal Antibody
Synonyms:   CDKN2; p33(CDK2)
UNIPROT ID:   P06493/P24941
Background:    Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2
Applications:    WB,IHC-P,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R06-1A5
MW:    Calculated MW: 34 kDa; Observed MW: 34 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk1/2 (Phospho-Thr14) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Cdk1/2 (Phospho-Thr14) in HepG2, U2OS, U251 lysates using Phospho-CDK1/2 (Thr14) antibody.
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