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PI3 Kinase p85 alpha (1C8) Mouse mAb

261375

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261375
Product Name:   Anti-PI3 Kinase p85 alpha (1C8) Mouse Monoclonal Antibody
Synonyms:   PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UNIPROT ID:   P27986
Background:    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
Immunogen:    Purified recombinant protein expressed in E.coli.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    1C8-2H4-9D9
MW:    Calculated MW: 84 kDa; Observed MW: 84 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemical analysis of paraffin-embedded Human tonsils using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded rat Liver Tissue using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of PI3 Kinase p85 alpha (1C8) in Jurkat, rat Brain, Raw264.7, Ramos, Raji lysates using PI3 Kinase p85 alpha (1C8) antibody

Western blot analysis of PI3 Kinase p85 alpha (1C8) in Hela, A549, HL-60, U2OS, C6 lysates using PI3 Kinase p85 alpha (1C8) antibody.
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