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VDAC1 Rabbit pAb

213281

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S213281
Product Name:   Anti-VDAC1 Rabbit Polyclonal Antibody
Synonyms:   PORIN; VDAC-1
UNIPROT ID:   P21796 (Gene Accession - NP_003365 )
Background:    This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.
Immunogen:    Synthetic peptide of human VDAC1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 2000-60000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 213281(VDAC1 Antibody) at a dilution of 1/13(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 213281(Anti-VDAC1 Antibody) at dilution 1/13.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Hela, 293T cell, Mouse brain tissue, 231, LNCAP, Jurkat, K562 cell lysates;
Primary antibody: 213281(VDAC1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second
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