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EFCAB7 Rabbit pAb

213091

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S213091
Product Name:   Anti-EFCAB7 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   A8K855 (Gene Accession - BC015814 )
Background:    Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia.
Immunogen:    Fusion protein of human EFCAB7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 213091(EFCAB7 Antibody) at a dilution of 1/75(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 213091(Anti-EFCAB7 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 and C2C12 cell lysates;
Primary antibody: 213091(EFCAB7 Antibody) at dilution 1/550;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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