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SHPK Rabbit pAb

212164

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S212164
Product Name:   Anti-SHPK Rabbit Polyclonal Antibody
Synonyms:   SHK; CARKL
UNIPROT ID:   Q9UHJ6 (Gene Accession - BC020543 )
Background:    The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements.
Immunogen:    Fusion protein of human SHPK
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 212164(SHPK Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 212164(Anti-SHPK Antibody) at dilution 1/45.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 212164(SHPK Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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