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PGRMC2 Rabbit pAb

211421

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S211421
Product Name:   Anti-PGRMC2 Rabbit Polyclonal Antibody
Synonyms:   DG6; PMBP
UNIPROT ID:   O15173 (Gene Accession - BC016692 )
Background:    PGRMC2 (progesterone receptor membrane component 2), also known as DG6 (steroid receptor protein DG6) or PMBP (progesterone membrane-binding protein), is a single pass membrane protein belonging to the cytochrome b5 family (MAPR (membrane associated progesterone receptor) subfamily). Expressed in sperm, PGRMC2 is believed to function as a steroid receptor and may participate in the progesterone-dependent sperm acrosome reaction. PGRMC2 shares approximately 50% overall sequence identity with its close relative PGRMC1. The loss of the gene encoding PGRMC2 is associated with metastasis in uterine endocervical adenocarcinomas, implicating a potential role of PGRMC2 in the suppression of metastasis of endocervical adenocarcinomas.
Immunogen:    Fusion protein of human PGRMC2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 211421(PGRMC2 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 211421(Anti-PGRMC2 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 211421(Anti-PGRMC2 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D122869(Anti-PGRMC2 Antibody) at dilution 1/70.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: RAW264.7 and NIH/3T3 cell lysates;
Primary antibody: 211421(PGRMC2 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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