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ACOT9 Rabbit pAb

210716

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S210716
Product Name:   Anti-ACOT9 Rabbit Polyclonal Antibody
Synonyms:   ACATE2; CGI-16; MTACT48; MT-ACT48
UNIPROT ID:   Q9Y305 (Gene Accession - NP_001028755 )
Background:    The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.
Immunogen:    Fusion protein of human ACOT9
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 210716(ACOT9 Antibody) at a dilution of 1/15(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 210716(Anti-ACOT9 Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: MCF7 cells, A172 cells;
Primary antibody: 210716(ACOT9 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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