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RRM1 Rabbit pAb

210551

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S210551
Product Name:   Anti-RRM1 Rabbit Polyclonal Antibody
Synonyms:   R1; RR1; RIR1
UNIPROT ID:   P23921 (Gene Accession - BC006498 )
Background:    This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region.
Immunogen:    Fusion protein of human RRM1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 210551(RRM1 Antibody) at a dilution of 1/15(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 210551(Anti-RRM1 Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 210551(Anti-RRM1 Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D121160(Anti-RRM1 Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane 1-2: Hela cells, 231 cells;
Primary antibody: 210551(RRM1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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