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HCK Rabbit pAb

210354

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S210354
Product Name:   Anti-HCK Rabbit Polyclonal Antibody
Synonyms:   JTK9; p59Hck; p61Hck
UNIPROT ID:   P08631 (Gene Accession - BC014435 )
Background:    The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.
Immunogen:    Fusion protein of human HCK
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 210354(HCK Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 210354(Anti-HCK Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 210354(Anti-HCK Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D120736(Anti-HCK Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cells;
Primary antibody: 210354(HCK Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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