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Human FZD10 Full Length Protein

11033

Size:50 μg Price:$ 4400 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:     11033
Product Name:   Human FZD10 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD350; FZ-10; Fz10; FzE7; hFz10
Target:    FZD10
UNIPROT ID:    Q9ULW2
Description:    Human FZD10 full length protein-synthetic nanodisc
Background:     A member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer.
Species/Host:    HEK293
Molecular Weight:    The human full length FZD10 protein has a MW of 65.3 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag FZD10-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with FZD10-Nanodisc is 2.854ng/ml.

Figure2. Human FZD10-Nanodisc, Flag Tag on SDS-PAGE
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