Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26160 |
| Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD |
| Description: Anti-IDH1(R132S) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin. |
| Applications:ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes IDH1(R132S) mutated protein, but not wild type IDH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant IDH1(R132S) and wild type proteins. Purified His-tagged IDH1(R132S) protein (lane 2) and wild type protein (lane 1) were blotted with Anti-IDH1(R132S) mouse antibody (Cat. #26160).
Immunofluorescence:
Immunofluorescence of cells expressing IDH1 proteins with Anti-IDH1(R132S) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1(R132S) plasmid (right column), then fixed and stained with Anti-IDH1(R132S) monoclonal antibody (Cat. #26160). |
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