Size：30 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26904 |
| Size: 30 µL |
| Gene Symbol: RHOA |
| Description: Anti-RhoA-GTP Mouse Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction. |
| Immunogen: Recombinant full length protein of active RhoA |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western Blot: 
IP/WB Analysis of Active RhoA Protein.
Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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