Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Rab35-GTP
Cat. #: 26922 |
Size: 30 µL |
Gene Symbol: RAB35 |
Description: Anti-Rab35-GTP Monoclonal Antibody |
Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. |
Immunogen: Recombinant full length protein of active Rab35 |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: Rab35 Activation Assay.Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase